CST, 61182S, P4HA1 (E3I3R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying P4HA1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, P4HA1 (E3I3R) Rabbit Monoclonal Antibody (CST #61182) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity P4HA1 (E3I3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total P4HA1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro358 of human P4HA1 isoform 2 protein. Background Prolyl 4-hydroxylase subunit alpha-1 (P4HA1) is a member of the prolyl 4-hydroxylase enzyme family, which is responsible for catalyzing the hydroxylation of specific proline residues within collagen polypeptide chains. This hydroxylation is a crucial step in collagen synthesis and maturation. The hydroxylated proline residues are essential for the stabilization of collagen's triple helical structure by facilitating the formation of hydrogen bonds. This structural stability is fundamental to collagen's mechanical properties and its ability to function as a structural scaffold in various tissues (1). The hydroxylation of proline residues in collagen is also critical for subsequent crosslinking reactions, which further strengthen the collagen fibrils and contribute to the overall stability of tissues. These crosslinks are important for tissue integrity, wound healing, and overall physiological function (1). P4HA1 has recently been identified in gene signatures associated with several tumors (2,3). Alternate Names 4-PH alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); P4HA; P4HA1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; prolyl 4-hydroxylase subunit alpha 1; Prolyl 4-hydroxylase subunit alpha-1; prolyl 4-hydroxylase, alpha polypeptide I Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG