CST, 61244S, RIF1 Antibody

Polyclonal Antibody for studying RIF1 mouse. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, RIF1 Antibody (CST #61244) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RIF1 Antibody recognizes endogenous levels of total RIF1 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1257 of mouse RIF1 protein. Antibodies are purified by peptide affinity chromatography. Background The Rap1 interacting factor 1 (RIF1) was originally identified as a regulator of telomere homeostasis in yeast and mammalian cells (1). Research studies show that RIF1 regulates the timing of eukaryotic DNA replication origin firing through its effect on chromatin architecture (2-4). Additional studies show that RIF1 is essential for regulating the repair of DNA double-strand breaks (DSBs). RIF1 is recruited to sites of DSBs by 53BP1 in response to DNA damage, and suppresses 5' end resection to favor the non-homologous end joining (NHEJ) pathway over homologous recombination (HR) repair (5-8). Oct-4 and Smad3 modulate RIF1 expression in mouse embryonic stem cells, and RIF1 may regulate embryonic stem cell stability during cell proliferation (9). Inhibition of ATR or CHK1 activity induces CDK1-mediated phosphorylation of RIF1 at serine 2205 (human)/serine 2153 (mouse), leading to firing of dormant origins of DNA replication during S phase (10,11). Alternate Names 5730435J01Rik; 6530403D07Rik; AU016181; AW549474; D2Ertd145; D2Ertd145e; mRif1; Rap1 interacting factor 1 homolog; Rap1 interacting factor 1 homolog (yeast); Rap1-interacting factor 1 homolog; replication timing regulatory factor 1; Rif1; Telomere-associated protein RIF1 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 266 SOURCE: Rabbit