CST, 61260S, XCL1/XCL2 Antibody

Polyclonal Antibody for studying XCL1. Validated for Western Blotting. Highly specific and rigorously validated in-house, XCL1/XCL2 Antibody (CST #61260) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity XCL1/XCL2 Antibody recognizes endogenous levels of total XCL1 and XCL2 proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro99 of human XCL1 protein. Antibodies are purified by peptide affinity chromatography. Background XCL1 (Lymphotactin, SCM-1 alpha, Chemokine (C motif) ligand 1) and XCL2 (SCM-1 beta, Chemokine (C motif) ligand 2) comprise the C chemokine family, lacking two of the four invariant cysteines normally found on chemokines (1,4). Their sequences differ by 2 amino acids and they are primarily expressed by CD8 T cells and NK cells (1-5). XCL1 and XCL2 act as ligands for the chemokine receptor XCR1 (1-5). XCR1-positive dendritic cells cross-present antigens to naïve CD8 T cells, priming them to become activated cytotoxic CD8 T cells (6,7). In addition, in mouse models, the XCL1-XCR1 signaling axis was shown to be involved in the formation of self-tolerance through the development of T cells within the thymus (8). Alternate Names ATAC; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; LPTN; LTN; Lymphotactin; Lymphotaxin; SCM-1; SCM-1-alpha; SCM-1a; SCM1; SCM1A; SCYC1; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); Small-inducible cytokine C1; X-C motif chemokine ligand 1; XC chemokine ligand 1; XCL1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 12 SOURCE: Rabbit