CST, 61358S, TIFA Antibody

Polyclonal Antibody for studying TIFA. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, TIFA Antibody (CST #61358) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TIFA Antibody recognizes endogenous levels of total TIFA protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro159 of human TIFA protein. Antibodies are purified by peptide affinity chromatography. Background TRAF-interacting protein with FHA domain-containing protein A (TIFA) is a cytosolic adaptor protein that is a critical node in an innate immune signaling axis that is activated upon detection of proinflammatory stimuli derived from a bacterial pathogen-associated molecular pattern metabolite, ADP-heptose (1-4). ADP-heptose binds and activates Alpha-protein kinase 1, which drives the phosphorylation-dependent oligomerization and activation of TIFA (5,6). Activated TIFA is then poised to shape the innate immune response by triggering TRAF6 oligomerization, IKK activation, and NF-κB-dependent proinflammatory gene transcription (1,2,5,7). Alternate Names MGC20791; Putative MAPK-activating protein PM14; Putative NF-kappa-B-activating protein 20; T2BP; T6BP; TIFA; TIFAA; TRAF interacting protein with forkhead associated domain; TRAF-interacting protein with a forkhead-associated domain; TRAF-interacting protein with FHA domain-containing protein A; TRAF-interacting protein with forkhead-associated domain; TRAF2 binding protein; TRAF2-binding protein; TRAF6 binding protein Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 21 SOURCE: Rabbit