CST, 61442SF, METTL3 (E3F2A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying METTL3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, METTL3 (E3F2A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #61442) is ready to ship. Product Usage Information This product is the carrier free version of product #86132. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 86132 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity METTL3 (E3F2A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total METTL3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human METTL3 protein. Background Methyltransferase-like protein 3 (METTL3) and methyltransferase-like protein 14 (METTL14) are the two catalytic subunits of an N6-methyltransferase complex that methylates adenosine residues in RNA (1). Methylation of adenosine residues regulates mRNA splicing, processing, translation efficiency, editing and stability, in addition to regulating primary miRNA processing, and is critical for proper regulation of the circadian clock, embryonic stem cell self-renewal, immune tolerance, response to various stimuli, meiosis and mouse fertility (2,3). In this complex, METTL3 functions as the catalytic methyltransferase subunit and METTL14 functions as the target recognition subunit by binding to RNA (4). In addition, the Wilms' tumor 1-associating protein (WTAP) functions as a regulatory subunit and is required for accumulation of the complex to nuclear speckles, which are sites of RNA processing (5). Several studies suggest a role for this complex in cancer. METTL3 expression is elevated in lung adenocarcinoma where it promotes growth, survival, and invasion of human lung cancer cells (6). In addition, WTAP is overexpressed in a number of different cancers and positively regulates cell migration and invasion in glioblastoma and cholangiocarcinoma (7,8). Alternate Names adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; hMETTL3; IME4; M6A; methyltransferase like 3; Methyltransferase-like protein 3; METTL3; MGC4336; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; MT-A70; MTA70; N6-adenosine-methyltransferase 70 kDa subunit; N6-adenosine-methyltransferase catalytic subunit; Spo8 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG