CST, 61521S, TRMT112 (F1A6F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TRMT112. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TRMT112 (F1A6F) Rabbit Monoclonal Antibody (CST #61521) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TRMT112 (F1A6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total TRMT112 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human TRMT112 protein. Background Multifunctional methyltransferase subunit TRM112-like protein (TRMT112) is an evolutionarily conserved 15 kDa protein that interacts with and stabilizes various methyltransferases involved in tRNA, rRNA, and protein methylation. While most studies have been in yeast, TRMT112 has been shown to interact with human methyltransferases involved in translation and ribosome biogenesis, including TRMT11, ALKBH8, HEMK2a, WBSCR22, and METTL5 (1-5). TRMT112 is also widely expressed during embryogenesis (6). The TRMT11-TRMT112 complex deposits 2-methylguanosine at position 10 of tRNA, while ALKBH8-TRMT112 modifies a uridine at position 34 of the anti-codon loop (5,7) Additionally, TRMT112 plays a role in translation termination by interacting with N6AMT1, causing methylation of the release factor eRF1 to allow for stop codon recognition (8,9). TRMT112 also contributes to ribosome biogenesis by interacting with and stabilizing WBSCR22, an 18s rRNA methyltransferase, and METTL5, the 18s rRNA m6a-methyltransferase (3,4,10). Alternate Names HSPC152; HSPC170; HSPC170 protein; hTrm112; Multifunctional methyltransferase subunit TRM112-like protein; TR112; TRM112; TRM112-like protein; TRMT11-2; TRMT112; tRNA methyltransferase 11-2 homolog; tRNA methyltransferase 11-2 homolog (S. cerevisiae); tRNA methyltransferase 112 homolog; tRNA methyltransferase subunit 11-2 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG