CST, 61572S, CEACAM8 (D2F5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CEACAM8. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CEACAM8 (D2F5U) Rabbit Monoclonal Antibody (CST #61572) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CEACAM8 (D2F5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total CEACAM8 protein. This antibody does not cross-react with CEACAM1 or CEACAM6 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CEACAM8 protein. Background Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, is a member of the CEA-related cell-adhesion molecule (CEACAM) family (1). CEACAMs bind to themselves and other family members to carry out numerous cellular functions, including proliferation, signaling, differentiation, tumor suppression, and survival (2). CEACAM8 is a single-chain, glycosylphosphatidylinositol (GPI)-anchored, highly glycosylated protein that under normal conditions is expressed almost exclusively on granulocytes (3). Heterophilic interactions of CEACAM8 with other surface molecules, such as CEACAM6, have been shown to be involved in regulating cell adhesion in a calcium-independent manner (4). As such, CEACAM8 has demonstrated utility as a marker of neutrophil and eosinophil activation (5,6) and in pathological conditions is shown to be highly expressed in primary myelofibrosis and acute lymphoblastic leukemias (7,8). Assessment of CEACAM8 positive tumor-infiltrating neutrophils has demonstrated value as a prognostic factor in multiple cancer types (9,10). Alternate Names Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen related cell adhesion molecule 8; Carcinoembryonic antigen-related cell adhesion molecule 8; CD66b; CD67; CD67 antigen; CEA cell adhesion molecule 8; CEACAM8; CEAM8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG