CST, 61586T, SAT1 (D1T7M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SAT. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SAT1 (D1T7M) Rabbit Monoclonal Antibody (CST #61586) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SAT1 (D1T7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total SAT1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp132 of human SAT1 protein. Background Spermidine/spermine N1-acetyltransferase 1 (SAT1) is a key enzyme in polyamine metabolism. It acts by acetylating spermidine and spermine using acetyl-coenzyme A (CoA), which alters their charge and facilitates their secretion (1,2). SAT1 activity is tightly controlled in cells, but increases quickly by excess polyamines. Its activity can also be induced by a number of other stimuli, such as oxidative stress, heat shock, insulin-like growth factor-I, and cytotoxins (2-4). Research studies have found that SAT1 is overexpressed in glioblastoma multiforme (GBM), which suggest that SAT1 may be a potential therapeutic target for GBM (5). Alternate Names DC21; Diamine acetyltransferase 1; diamine N-acetyltransferase 1; epididymis secretory sperm binding protein; KFSD; KFSDX; Polyamine N-acetyltransferase 1; Putrescine acetyltransferase; SAT; SAT1; Spermidine/spermine N(1)-acetyltransferase 1; spermidine/spermine N1-acetyltransferase 1; spermidine/spermine N1-acetyltransferase alpha; SSAT; SSAT-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG