CST, 61637S, OX40 (E9U7O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TNFRSF4. Validated for WB,IP,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, OX40 (E9U7O) Rabbit Monoclonal Antibody (CST #61637) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 IHC Leica Bond: 1:400 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Immunocytochemistry): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 76367 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity OX40 (E9U7O) Rabbit Monoclonal Antibody recognizes endogenous levels of total OX40 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln264 of human OX40 protein. Background OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor superfamily that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif (1,2). OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells (3-7). The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway (2,8). OX40 signaling through Akt can also enhance TCR signaling directly (9). Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and autoimmune diseases (10). Additional research studies show that OX40 agonists augment anti-tumor immunity in several cancer types (11,12). Alternate Names ACT35; ACT35 antigen; ATC35 antigen; CD134; CD134 antigen; IMD16; lymphoid activation antigene ACT35; OX40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNF receptor superfamily member 4; TNFRSF4; TNR4; Tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; TXGP1L Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35-50 Source/Isotype: Rabbit IgG