CST, 61751SF, Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying cre recombinase. Validated for WB,IHC,IF,IF,F. Highly specific and rigorously validated in-house, Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #61751) is ready to ship. Product Usage Information This product is the carrier free version of product #15036. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 15036 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Cre Recombinase (D7L7L) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes transfected and transgenic levels of total Cre recombinase protein. Species Reactivity: All Species Expected Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of bacteriophage-P1 Cre recombinase protein. Background Cre recombinase is a bacteriophage-P1 enzyme required for maintenance of the phage genome as a monomeric plasmid in the lysogenic state (1,2). This enzyme mediates a site-specific recombination between two 34-base-pair loxP sites. This reaction can be carried out , indicating that it does not require accessory factors (3). The Cre/Lox system has been used for a number of and applications, including targeted gene deletions (4) and gene-specific humanized animal models (5). Resolution of the crystal structure of the Cre-Lox complex revealed that two Cre molecules interact with a single Lox site (6). Alternate Names cre; cre recombinase; cyclization recombinase; Recombinase cre Specification REACTIVITY: All SENSITIVITY: Transfected Only MW (kDa): 37 Source/Isotype: Rabbit IgG