CST, 61874W2, Brachyury (D2Z3J) Rabbit Monoclonal Antibody (SignalFlex™ Pacific Blue™ Conjugate)

Monoclonal Antibody for studying Brachyury. Highly specific and rigorously validated in-house, Brachyury (D2Z3J) Rabbit Monoclonal Antibody (SignalFlex^™ Pacific Blue^™ Conjugate) (CST #61874) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Brachyury (D2Z3J) Rabbit mAb (SignalFlex™ Pacific Blue™ Conjugate) recognizes endogenous levels of total Brachyury protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro256 of human Brachyury protein. Background Brachyury protein, encoded by the gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis (1). In the mouse, brachyury is necessary for mesodermal morphogenetic cell movements during gastrulation. Brachyury mutant mice die and display deficient mesoderm formation including an abnormal notochord, missing posterior somites, and a reduced allantois (2). Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors (3,4). A common polymorphism in the human gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida (5,6). Alternate Names BRAC; Brachyury; Brachyury protein; MGC104817; OTTHUMP00000017588; Protein T; SAVA; T; T brachyury; T brachyury transcription factor; T brachyury-like; T-box transcription factor T; T, brachyury homolog; T, brachyury homolog (mouse); TBXT; TFT; transcription factor T Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG