CST, 61906S, Goat Anti-Mouse IgG2a Antibody (AP Conjugate)

Secondary Antibody for studying in the research area. Product Usage Information Recommended Antibody Dilutions: 1:2000 - 1:8000 Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability. Storage Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Do not aliquot the antibody. Specificity / Sensitivity Goat Anti-Mouse IgG2a Antibody (AP Conjugate) detects the heavy chain of mouse IgG2a but does not cross-react with other mouse IgG subclasses, mouse IgM, or mouse IgA. This antibody conjugate has minimal cross-reaction with human, bovine, and horse serum proteins. This antibody conjugate may cross-react with immunoglobulins from other species. Species Reactivity: Mouse Source / Purification Goat Anti-Mouse IgG2a Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG2a. The Anti-Mouse IgG2a antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Background The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time. Specification REACTIVITY: M Source/Isotype: Goat