CST, 61921T, Gasdermin C (F9R3M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Gasdermin C. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Gasdermin C (F9R3M) Rabbit Monoclonal Antibody (CST #61921) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Gasdermin C (F9R3M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Gasdermin C protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro125 of human Gasdermin C protein. Background The gasdermin family, which includes GSDMA, GSDMB, GSDMC, GSDMD, and GSDME, has been shown to play a role in inflammation and cell death. Gasdermin D has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). Overexpression of Gasdermin C (GSDMC) in several types of cancer has been associated with disease progression and prognosis (8-13). Cleavage of GSDMC can be induced by caspase-8, with the N-terminal fragment initiating pyroptosis (14,15). Alternate Names gasdermin C; Gasdermin-C; GSDMC; Melanoma-derived leucine zipper-containing extranuclear factor; melanoma-derived leucine zipper, extra-nuclear factor; MLZE Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 59 Source/Isotype: Rabbit IgG