CST, 61957SF, Phospho-HS1 (Tyr378/397) (D12C1) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying HS1 (Tyr397) phosphate. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized),ELISA+. Highly specific and rigorously validated in-house, Phospho-HS1 (Tyr378/397) (D12C1) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #61957) is ready to ship. Product Usage Information This product is the carrier free version of product #8714. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8714 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-HS1 (Tyr378/397) (D12C1) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of HS1 protein only when phosphorylated at Tyr378 or Tyr397. Mouse cross-reactivity is determined by ELISA. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein. Background HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7). HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10). Alternate Names cortactin-like; CTTNL; HCLS1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS1; LckBP1; p75 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG