CST, 62115S, DHCR7 (F7I5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DHCR7. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, DHCR7 (F7I5B) Rabbit Monoclonal Antibody (CST #62115) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity DHCR7 (F7I5B) Rabbit mAb recognizes endogenous levels of total DHCR7 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DHCR7 protein. Background 7-dehydrocholesterol reductase (DHCR7) catalyzes the conversion of 7-dehydrocholesterol (7-DHC) to cholesterol, the last step of cholesterol biosynthesis. Studies show that DHCR7 expression is downregulated in macrophages upon viral infection. DHCR7 deficiency activates the PI3K-AKT3 pathway. AKT3 phosphorylates interferon regulatory factor 3 (IRF3) at Ser385, leading to IRF3 activation and increased type I interferon (IFN-I) production in macrophages (1). In addition, DHCR7 has proferroptotic activity. Its substrate, 7-DHC, suppresses ferroptosis by inhibiting phospholipid peroxidation. Blocking 7-DHC biosynthesis enhances ferroptosis of cancer cells (2,3). Alternate Names 7-dehydrocholesterol reductase; 7-DHC reductase; D7SR; delta-7-dehydrocholesterol reductase; Delta7-sterol reductase; DHCR7; Putative sterol reductase SR-2; SLOS; sterol delta-7-reductase; Sterol Delta(7)-reductase; Sterol reductase SR-2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 40 Source/Isotype: Rabbit IgG