CST, 62133T, CD54/ICAM-1 (VF27-516) Mouse Monoclonal Antibody

Monoclonal Antibody for studying CD54/ICAM1. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD54/ICAM-1 (VF27-516) Mouse Monoclonal Antibody (CST #62133) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:500 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Flow Cytometry (Live): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD54/ICAM-1 (VF27-516) Mouse Monoclonal Antibody recognizes endogenous levels of total CD54/ICAM-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with SDS denatured, formalin fixed cultured COLO-38 human melanoma cells. Background Intercellular cell adhesion molecule-1 (CD54 or ICAM-1) is a cell surface glycoprotein that belongs to the immunoglobulin superfamily (IgSF) of adhesion molecules. CD54 is expressed at low levels in diverse cell types, and is induced by cytokines (TNF-α, interleukin-1) and bacterial lipopolysaccharide (1). Apical localization of CD54 on endothelial cells (or basolateral localization on epithelial cells) is a prerequisite for leukocyte trafficking through the endothelial (or epithelial) barrier (1). Apical expression of CD54 on epithelial cells mediates pathogen invasion as well as host defense, a pattern also observed in tumors (1). CD54 also functions as a co-stimulator on antigen presenting cells, binding to its receptor LFA-1 (leukocyte function-associated antigen-1) on the surface of T cells during antigen presentation (2). Cross-linking of CD54 or binding to its ligand triggers activation of Src family kinases and the Rho/ROCK pathway (3-7). Phosphorylation on Tyr485 of CD54 is required for its association with SHP-2 (5). SHP-2 seems essential for CD54-induced Src activation (7). Alternate Names BB2; CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; human rhinovirus receptor; ICAM-1; ICAM1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Major group rhinovirus receptor; P3.58 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1