CST, 62184S, CBF beta (D4N2N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CBFB. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, CBF beta (D4N2N) Rabbit Monoclonal Antibody (CST #62184) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity CBF beta (D4N2N) Rabbit Monoclonal Antibody recognizes endogenous levels of total CBFβ protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn14 of human CBFβ protein. Background Core-binding factor subunit β (CBFβ) is a non-DNA binding transcription factor subunit that associates with and regulates the DNA binding activity of RUNX1, RUNX2, and RUNX3 (1). CBFβ is ubiquitously expressed and has been implicated in a variety of developmental processes including hematapoiesis, T cell development, chondrogenesis, and bone formation (2-7). In addition, investigators have identified CBFβ as one of the most frequently translocated genes in leukemia (8) and research studies have found it to be required for HIV immune evasion (9,10). CBFβ interacts with the viral protein VIF and triggers assembly of a ubiquitin ligase complex that targets the retroviral inhibitor APOBEC3G for degradation (9,10). Alternate Names CBF-beta; CBFB; core-binding factor beta subunit; Core-binding factor subunit beta; core-binding factor, beta subunit; PEA2-beta; PEBB; PEBP2-beta; PEBP2B; polyomavirus enhancer binding protein 2, beta subunit; Polyomavirus enhancer-binding protein 2 beta subunit; SL3-3 enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG