CST, 62257S, DCLK1/DCAMKL1 (D2U3L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DCAMKL1. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, DCLK1/DCAMKL1 (D2U3L) Rabbit Monoclonal Antibody (CST #62257) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:300 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 10208 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity DCLK1/DCAMKL1 (D2U3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total DCLK1/DCAMKL1 protein. The identity of the faint lower molecular weight bands is not known. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DCLK1/DCAMKL1 protein. Background Doublecortin-like kinase 1 (DCLK1, DCAMKL1) is a serine/threonine kinase that belongs to the CaM kinase family and shares homology with the neuronal microtubule binding protein doublecortin. DCLK1 is thought to be involved in calcium signaling pathways controlling neuronal development in the embryonic brain (1,2). The kinase also functions in the mature nervous system and is highly expressed in regions of active neurogenesis in the neocortex and cerebellum (3,4). Research studies suggest that the DCLK1 kinase is highly expressed in subpopulations of cells within the colon and gastric epithelium and in the pancreas (5-8). The nature of these cell populations, whether normal, stem-like, or tumor-initiating, is unclear. Alternate Names CL1; CLICK1; DCAMKL1; DCDC3A; DCLK; DCLK1; doublecortin and CaM kinase-like 1; Doublecortin domain-containing protein 3A; doublecortin like kinase 1; Doublecortin-like and CAM kinase-like 1; Doublecortin-like kinase 1; KIAA0369; Serine/threonine-protein kinase DCLK1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 82 Source/Isotype: Rabbit IgG