CST, 62265S, SMARCD3/BAF60C (D6F1S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMARCD3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SMARCD3/BAF60C (D6F1S) Rabbit Monoclonal Antibody (CST #62265) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SMARCD3/BAF60C (D6F1S) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMARCD3/BAF60C protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human SMARCD3/BAF60C protein. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). SMARCD3/BAF60C is essential for the development of the skeletal and cardiac muscle (10,11). Knockdown of SMARCD3/BAF60C using RNAi in mouse embryos leads to defects in heart development as well as abnormalities in the development of the cardiac and skeletal muscle, mimicking the cardiac defects observed in congenital heart disease (12). In addition, SMARCD3/BAF60C is part of the lipoBAF complex that remodels the chromaitn structure to activate lipogenic genes, promoting lipogenesis in response to feeding and insulin (13). Alternate Names 60 kDa BRG-1/Brm-associated factor subunit C; 60kDa BRG-1/Brm associated factor subunit c; BAF60C; BRG1-associated factor 60C; chromatin remodeling complex BAF60C subunit; CRACD3; mammalian chromatin remodeling complex BRG1-associated factor 60C; MGC111010; Rsc6p; SMARCD3; SMRD3; SWI/SNF complex 60 kDa subunit C; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3, isoform 1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 3; Swp73-like protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG