CST, 62328S, CSDE1 Antibody

Polyclonal Antibody for studying CSDE1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CSDE1 Antibody (CST #62328) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CSDE1 Antibody recognizes endogenous levels of total CSDE1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CSDE1 protein. Antibodies are purified by peptide affinity chromatography. Background Originally described as the product of an active transcription unit located upstream of the  gene (1), CSDE1 (cold shock domain-containing protein E1) is a highly conserved RNA-binding protein containing five cold-shock domains (2,3). CSDE1 is mostly localized in the cytoplasm where it interacts with distinct protein complexes involved in the post-transcriptional regulation of numerous mRNAs in a dynamic and complex manner (4). CSDE1 plays a role in maintaining the undifferentiated state of human embryonic stem cells (hESCs), and its loss results in accelerated neural differentiation and neurogenesis, partially via post-transcriptional regulation of fatty acid binding protein 7 ( ) and vimentin ( ) mRNAs (5). In human melanoma, increased CSDE1 protein expression has been shown to modulate the levels of pro-oncogenic factors such as vimentin or RAC1 and tumor suppressors such as PTEN, thus promoting tumor invasion and metastasis (6). Alternate Names cold shock domain containing E1; cold shock domain containing E1, RNA binding; cold shock domain containing E1, RNA-binding; Cold shock domain-containing protein E1; CSDE1; D1S155E; DKFZp779B0247; DKFZp779J1455; FLJ26882; KIAA0885; N-ras upstream gene protein; NRAS-related; NRU; Protein UNR; RP5-1000E10.3; UNR; upstream of NRAS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 89 SOURCE: Rabbit