CST, 62370S, SMRT (D8D2L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SMRT. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SMRT (D8D2L) Rabbit Monoclonal Antibody (CST #62370) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity SMRT (D8D2L) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMRT protein. This antibody does not cross-react with NCoR1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein corresponding to residues near the amino terminus of human SMRT protein. Background The most well characterized nuclear receptor corepressors are NCoR1 (nuclear receptor corepressor) and its close paralog NCoR2, also know as SMRT (silencing mediator for retinoic acid and thyroid hormone receptors) (1,2). NCoR1 and SMRT function to transcriptionally silence various unliganded, DNA bound non-steroidal nuclear receptors by serving as a large molecular scaffold that bridges the receptors with multiple chromatin remodeling factors that repress nuclear receptor-mediated gene transcription, in part, through deacetylation of core histones surrounding target promoters. Indeed, the N-terminal portion of NCoR1 and SMRT possess multiple distinct transcriptional repression domains (RDs) responsible for the recruitment of additional components of the corepressor complex such as HDACs, mSin3, GPS2, and TBL1/TBLR1. In between the RDs lies a pair of potent repressor motifs known as SANT motifs (SWI3, ADA2, N-CoR, and TFIIIB), which recruit HDAC3 and histones to the repressor complex in order to enhance HDAC3 activity (3). The C-terminal portion of NCoR1 and SMRT contain multiple nuclear receptor interaction domains (NDs), each of which contains a conserved CoRNR box (or L/I-X-X-I/V-I) motif that allow for binding to various unliganded nuclear hormone receptors such as thyroid hormone (THR) and retinoic acid (RAR) receptors (4,5). Alternate Names CTG repeat protein 26; CTG26; FLJ25011; N-CoR2; NCOR2; nuclear receptor co-repressor 2; Nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; Silencing mediator of retinoic acid and thyroid hormone receptor; SMAP270; SMRT; SMRTE; SMRTE-tau; T3 receptor-associating factor; Thyroid-, retinoic-acid-receptor-associated corepressor; TNRC14; TRAC; TRAC-1; TRAC1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 270 Source/Isotype: Rabbit IgG