CST, 6248S, MYH (D13D4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MYH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MYH (D13D4) Rabbit Monoclonal Antibody (CST #6248) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MYH (D13D4) Rabbit Monoclonal Antibody recognizes endogenous levels of total MYH protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MYH protein. Background Base excision repair (BER) proteins catalyze the removal of incorrect or damaged bases, including oxidized bases, from DNA. N-glycosylases specific to a given lesion remove the incorrect base as the first step in BER. MYH is the mammalian ortholog of MutY, a DNA glycosylase that catalyzes the removal of 8-oxoG:A mismatches (1). Several MYH isoforms have been detected in human cells localizing to either the nucleus or the mitochondria (2). MYH interacts with DNA repair proteins and localizes to DNA damage foci after oxidative damage (3). Research studies have shown that mutations in the corresponding MYH gene are associated with human gastric (4) and colorectal (5-7) cancers. Alternate Names A/G-specific adenine DNA glycosylase; Adenine DNA glycosylase; CYP2C; hMYH; MGC4416; mutY DNA glycosylase; MutY homolog; mutY homolog (E. coli); mutY-like protein; MUTYH; MYH Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55 Source/Isotype: Rabbit IgG