CST, 62622T, FITM2 (E5O8W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FITM2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FITM2 (E5O8W) Rabbit Monoclonal Antibody (CST #62622) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FITM2 (E5O8W) Rabbit Monoclonal Antibody recognizes endogenous levels of total FITM2 protein. This antibody detects a 60 kDa band of unknown origin in some cell lines. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln135 of human FITM2 protein. Background FITM2 (FIT2, C20orf142, acyl-coenzyme A diphosphatase FITM2, fat storage-inducing transmembrane protein 2) is a member of the FIT family of proteins that localize to the endoplasmic reticulum (ER) membrane (1-6). FITM2 is highly expressed by adipocytes and is essential for ER lipid metabolism and lipid droplet formation (1-6). FITM2 maintains ER homeostasis by hydrolyzing fatty acyl-CoA to acyl 4′-phosphopantetheine (6). Alternate Names Acyl-coenzyme A diphosphatase FITM2; C20orf142; dJ881L22.2; fat inducing transcript 2; fat storage inducing transmembrane protein 2; Fat storage-inducing transmembrane protein 2; Fat-inducing protein 2; fat-inducing transcript 2; FIT2; FITM2; MGC30135; SIDDIS Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 22 Source/Isotype: Rabbit IgG