CST, 62696S, KIF5B (E4A5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KIF5B. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, KIF5B (E4A5A) Rabbit Monoclonal Antibody (CST #62696) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity KIF5B (E4A5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total KIF5B protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly408 of human KIF5B protein. Background Kinesin superfamily proteins (KIFs) are molecular motors that drive directional, microtubule-dependent intracellular transport of membrane-bound organelles and other macromolecules (e.g., proteins, nucleic acids). The intracellular transport functions of KIFs are fundamentally important for a variety of cellular functions, including mitotic and meiotic division, motility/migration, hormone and neurotransmitter release, and differentiation (1-4). Disruptions to KIF-mediated intracellular transport have been linked with a variety of pathologies, ranging from tumorigenesis to defects in higher order brain function such as learning and memory (4-6). KIF5 consists of three family members referred to as KIF5A, KIF5B, and KIF5C (7). KIF5A and KIF5C are specifically expressed in neurons whereas KIF5B is expressed ubiquitously (7-10). Targeted disruption of the gene resulted in embryonic lethality (11). These and other studies demonstrate that KIF5B plays an important role in the localization and distribution of major organelles, including the mitochondria and lysosome, and can contribute to autophagy (11-13). In addition, gene rearrangements involving KIF5B fusions to ALK and RET have been identified as drivers for lung cancer and other malignancies (14-18). Alternate Names Conventional kinesin heavy chain; epididymis secretory protein Li 61; HEL-S-61; KIF5B; kinesin 1 (110-120kD); kinesin family member 5B; kinesin heavy chain; Kinesin-1 heavy chain; KINH; KNS; KNS1; Ubiquitous kinesin heavy chain; UKHC Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG