CST, 62742SF, Desmin (F5V4I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Desmin. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, Desmin (F5V4I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #62742) is ready to ship. Product Usage Information This product is the carrier free version of product #73348. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 73348 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Desmin (F5V4I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total desmin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg429 of human desmin protein. Background The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments, and microtubules. Major types of intermediate filaments are distinguished and expressed in particular cell types: cytokeratins (epithelial cells), glial fibrillary acidic protein or GFAP (glial cells), desmin (skeletal, visceral, and certain vascular smooth muscle cells), vimentin (mesenchyme origin), and neurofilaments (neurons). GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). Vimentin is present in sarcomas but not carcinomas, and its expression is examined relative to other markers to distinguish between the two forms of neoplasm (3). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs. The desmin cytoskeleton provides a connection among myofibrils, organelles, and the cytoskeleton (4). Desmin knockout mice develop cardiomyopathy and skeletal and smooth muscle defects (5). In humans, desmin-related myopathies might be caused by mutations in the corresponding desmin gene or in proteins with which desmin interacts, including αB-crystallin and synemin. Disorganized desmin filaments and the accumulation of protein aggregates composed predominantly of desmin characterize desmin-related myopathies (reviewed in 6,7). Alternate Names cardiomyopathy, dilated 1F (autosomal dominant); CDCD3; CMD1I; CSM1; CSM2; DES; DESM; Desmin; epididymis secretory sperm binding protein; FLJ12025; FLJ39719; FLJ41013; FLJ41793; intermediate filament protein; LGMD1D; LGMD1E; LGMD2R Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 53 Source/Isotype: Rabbit IgG