CST, 62759T, SLAMF3/CD229 (E5E8A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD229. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SLAMF3/CD229 (E5E8A) Rabbit Monoclonal Antibody (CST #62759) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SLAMF3/CD229 (E5E8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLAMF3/CD229 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the intracellular domain of human SLAMF3/CD229 protein. Background Signaling lymphocytic activation molecule family 3 (SLAMF3/CD229) is a type-I transmembrane glycoprotein belonging to the SLAM family of immunoreceptors. Research studies have demonstrated that SLAMF3/CD229 is a homophilic, self-ligating receptor and expression is largely restricted to the surface of T cells and B cells (1,2). The extracellular domain of SLAMF3/CD229 contains a duplicated immunoglobulin (Ig)-like variable-type domain and a duplicated Ig-like C2-type domain, while the cytoplasmic domain is subject to alternative splicing and contains multiple potential tyrosine phosphorylation sites capable of recruiting signaling molecules such as EAT-2 and SAP (1,3,4). Research studies have shown that SLAMF3/CD229 is also highly overexpressed by multiple myeloma plasma cells and drives intracellular signaling cascades that promote plasma cell proliferation and survival (5-7). Alternate Names CD229; Cell surface molecule Ly-9; cell-surface molecule Ly-9; hly9; LY9; Lymphocyte antigen 9; mLY9; Signaling lymphocytic activation molecule 3; SLAM family member 3; SLAMF3; T-lymphocyte surface antigen Ly-9 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120, 100 Source/Isotype: Rabbit IgG