CST, 62765T, MTAP (A8N9F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTAP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MTAP (A8N9F) Rabbit Monoclonal Antibody (CST #62765) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MTAP (A8N9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTAP protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly28 of human MTAP protein. Background MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5'-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1,2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors, including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma, and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6). Alternate Names 5'-methylthioadenosine phosphorylase; BDMF; c86fus; DMSFH; DMSMFH; epididymis luminal protein 249; epididymis secretory sperm binding protein; HEL-249; LGMBF; MeSAdo phosphorylase; methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAP; MTAPase; S-methyl-5'-thioadenosine phosphorylase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 29 Source/Isotype: Rabbit IgG