CST, 62815SF, MX1 (D3W7I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MX1. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, MX1 (D3W7I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #62815) is ready to ship. Product Usage Information This product is the carrier free version of product #37849. All were was generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 37849 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity MX1 (D3W7I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total MX1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu292 of human MX1 protein. Background MX1 (Myxovirus resistance protein 1/MxA) is an interferon-inducible antiviral protein that confers resistance to RNA viruses (1-4). MX1 has GTPase activity, and GTP-bound MX1 adopts a conformation that enables interaction with viral nucleocapsids (5-7). This interaction blocks transport of viral nucleocapsids to the nucleus, which prevents transcription of the viral genome (7,8). Structural studies suggest that the antiviral activity of MX1 involves the formation of MX1 oligomeric rings around viral nucleocapsids (9-12). Alternate Names IFI-78K; IFI78; interferon inducible protein p78; Interferon-induced GTP-binding protein Mx1; Interferon-induced GTP-binding protein Mx1, N-terminally processed; Interferon-induced protein p78; interferon-inducible protein p78; Interferon-regulated resistance GTP-binding protein MxA; lncMX1-215; MX; MX dynamin like GTPase 1; MX1; MxA; Myxoma resistance protein 1; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78 (mouse); Myxovirus resistance protein 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 76 Source/Isotype: Rabbit IgG