CST, 62823S, SARS-CoV-2 EndoRNAse Antibody

Polyclonal Antibody for studying endoRNAse-pp1ab SARS-CoV-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, SARS-CoV-2 EndoRNAse Antibody (CST #62823) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity SARS-CoV-2 EndoRNAse Antibody recognizes transfected levels of total SARS-CoV-2 endoRNAse protein. Species Reactivity: Virus Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of SARS-CoV-2 endoRNAse protein. Antibodies are purified by peptide affinity chromatography. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). SARS-CoV-2 Endonuclease, also called Nsp15, is translated from ORF1b. It is a uridine specific endoribonuclease that is thought to specifically cleave polyuridine RNA sequence at the 5' end of the template negative strand. Since polyuridine RNA can trigger the PAMP response, this action of the nuclease may block interferon production (3). It is also proposed to prevent accumulation of dsRNA intermediates and the formation of stress granules which are cell anti-viral responses (4). Together, these activities may prevent activation of host cell immune responses. Alternate Names endoRNAse; endoRNAse-pp1ab; NendoU; non-structural protein 15; nsp15; Nsp15 (6453-6798); SARS-CoV-2 nsp15; Uridylate-specific endoribonuclease Specification REACTIVITY: Vir SENSITIVITY: Transfected Only MW (kDa): 45 SOURCE: Rabbit