CST, 62825S, Phospho-SHP-1 (Ser591) (F3Y8A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SHP-1 (Ser591) phosphate. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-SHP-1 (Ser591) (F3Y8A) Rabbit Monoclonal Antibody (CST #62825) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-SHP-1 (Ser591) (F3Y8A) Rabbit Monoclonal Antibody recognizes endogenous levels of SHP-1 protein only when phosphorylated at Ser591. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser591 of human SHP-1 protein. Background SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain (1). SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EPORs) to negatively regulate hematopoietic growth (2). Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation (3). Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation (4). Because SHP-1 downregulates various proliferation pathways, SHP-1 is considered a potential tumor suppressor and angiogenesis regulator (5,6). SHP-1 can be phosphorylated by PKC at its C-terminal Ser591. The phosphorylation at Ser591 inhibits SHP-1 phosphatase activity (7). Alternate Names HCP; HCPH; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; protein tyrosine phosphatase non-receptor type 6; protein tyrosine phosphatase, non-receptor type 6; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; PTP1C; PTPN6; SH-PTP1; SHP-1; SHP-1L; SHP1; Tyrosine-protein phosphatase non-receptor type 6 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 68 Source/Isotype: Rabbit IgG