CST, 62834T, IRF-4 (E8H3S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRF-4. Validated for WB,IP,IHC,IHC,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IRF-4 (E8H3S) Rabbit Monoclonal Antibody (CST #62834) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 IHC Leica Bond: 1:50 - 1:200 Immunohistochemistry (Paraffin): 1:50 - 1:200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56471 . Protocol Available protocols: Western Blotting, Immunoprecipitation, IHC Leica Bond, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IRF-4 (E8H3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRF-4 protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human IRF-4 protein. Background Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2). IRF-4 was independently cloned by three groups and demonstrated to have roles in different contexts of lymphoid regulation (3-5). First, IRF-4 (Pip) was found to associate with PU.1, a hematopoietic specific member of the ETS family, and to regulate the expression of B-cell specific genes (3). Second, it was characterized as a lymphoid-specific member of the IRF family (LSIRF) able to bind to ISRE (4). Third, it was identified in activated T cells as a factor that binds to the promoter of the interleukin-5 gene (ICSAT), and it was shown to repress gene activation induced by IFN (5). IRF-4 is expressed in all stages of B cell development and in mature T cells, and is inducible in primary lymphocytes by antigen mimetic stimuli such as concanavalin A, CD3 crosslinking, anti-IgM and PMA treatment (4,5). Mice deficient in IRF-4 show normal distribution of B and T lymphocytes at 4 to 5 weeks, but later develop progressive generalized lymphadenopathy, suggesting a role for IRF-4 in the function and homeostasis of mature B- and T-lymphocytes (6). Alternate Names Interferon regulatory factor 4; IRF-4; IRF4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5; SHEP8 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 51 Source/Isotype: Rabbit IgG