CST, 62935S, BAF Antibody

Polyclonal Antibody for studying BAF. Validated for Western Blotting. Highly specific and rigorously validated in-house, BAF Antibody (CST #62935) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity BAF Antibody recognizes endogenous levels of total BAF protein. This antibody detects a 60 kDa band of unknown origin. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro14 of human BAF protein. Antibodies are purified by peptide affinity chromatography. Background Barrier-to-autointegration factor (BAF or BANF1) plays a major role in the lifecycle of retroviruses, where it inhibits the reverse transcribed viral genome from integrating into itself (1-3). BAF exists as a dimer in solution, and serves as a bridge for double-stranded DNA in an ordered nucleoprotein complex (4). BAF interacts with LEM-domains of lamin binding proteins emerin and LAP2, and serves to help reform the nuclear envelope during telophase (5-7). The binding of BAF to its various partners is inhibited by Ser4 phosphorylation, which is deposited by vaccinia-related kinases (VRKs) and occurs during interphase and mitosis (8,9). Certain viral genomes encode kinases extremely similar to VRK to prevent BAF from binding to their DNA, impeding viral replication (10,11). BAF has been implicated in various tumor types and is associated with poor prognosis (12-14). Alternate Names BAF; BAF nuclear assembly factor 1; BANF1; barrier to autointegration factor 1; Barrier-to-autointegration factor; Barrier-to-autointegration factor, N-terminally processed; BCRG1; BCRP1; Breakpoint cluster region protein 1; D14S1460; MGC111161; NGPS Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 10, 11 SOURCE: Rabbit