CST, 63079S, ILF2 (E4P4P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ILF2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, ILF2 (E4P4P) Rabbit Monoclonal Antibody (CST #63079) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity ILF2 (E4P4P) Rabbit Monoclonal Antibody recognizes endogenous levels of total ILF2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human ILF2 protein. Background ILF2 and ILF3, also known as NF45 and NF90 respectively, form a double-stranded RNA binding heterodimer that serves many different biological functions. ILF2 and ILF3 were originally discovered as proteins that can bind and regulate the antigen receptor response element of the IL2 promoter to contribute to T cell activation (1,2). ILF2 and ILF3 play important roles in DNA damage response and non-homologous end joining (NHEJ) by interacting with DNA-PK (3,4). The heterodimer can also bind and negatively regulate microRNAs (5,6). Biogenesis of the 60S ribosomal subunit has been shown to be dependent on the double-stranded RNA-binding domains of ILF3 (7). Additionally, ILF2 and ILF3 can mediate rDNA transcription (8). ILF2 and ILF3 also play a role in the life cycle of many different viruses, including dengue and hepatitis C (9-11). Alternate Names ILF2; interleukin enhancer binding factor 2; interleukin enhancer binding factor 2, 45kD; interleukin enhancer binding factor 2, 45kDa; Interleukin enhancer-binding factor 2; MGC8391; NF45; Nuclear factor of activated T-cells 45 kDa; nuclear factor of activated T-cells, 45-kDa; PRO3063 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 45 Source/Isotype: Rabbit IgG