CST, 63182S, UBE2G2 (D8Z4G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UBE2G2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, UBE2G2 (D8Z4G) Rabbit Monoclonal Antibody (CST #63182) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity UBE2G2 (D8Z4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBE2G2 protein. This antibody does not cross-react with UBE2G1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBE2G2 protein. Background Ubiquitin (Ub) is a conserved polypeptide that is covalently linked to many cellular proteins through the process of ubiquitination, which targets proteins for degradation by the 26S proteasome. Three enzymatic components are involved in the protein ubiquitination cascade. Ubiquitin is first activated by forming a thioester complex with an E1 ubiquitin-activating enzyme. Activated ubiquitin is subsequently transferred to an E2 ubiquitin-carrier protein, and then from the E2 to an E3 ubiquitin ligase for final delivery to the ε-amino group of the target protein lysine residue (1-3). The ubiquitin-conjugating enzyme E2 G2 (UBE2G2, UBC7) is a ubiquitously expressed E2 enzyme and critical component of the endoplasmic reticulum-associated degradation pathway (ERAD) (4). Research studies demonstrate that UBE2G2 forms homodimers and preassembles K48-linked poly-Ub chains at its active site (5-8). The association of Ub-charged UBE2G2 molecules with the ER-resident E3 ligase AMFR (gp78) is required for Ub chain transfer and efficient removal of misfolded or aggregated proteins through the ERAD pathway (9,10). Alternate Names E2 ubiquitin-conjugating enzyme G2; UB2G2; UBC7; UBE2G2; Ubiquitin carrier protein G2; ubiquitin conjugating enzyme 7; ubiquitin conjugating enzyme E2 G2; ubiquitin conjugating enzyme G2; Ubiquitin-conjugating enzyme E2 G2; ubiquitin-conjugating enzyme E2G 2 (homologous to yeast UBC7); ubiquitin-conjugating enzyme E2G 2 (UBC7 homolog, yeast); Ubiquitin-protein ligase G2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 19 Source/Isotype: Rabbit IgG