CST, 63227T, YY1 (D3D4Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying YY1. Validated for WB,IP,IF,F,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, YY1 (D3D4Q) Rabbit Monoclonal Antibody (CST #63227) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:100 Chromatin IP: 1:100 Chromatin IP-seq: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP, Chromatin IP-seq Specificity / Sensitivity YY1 (D3D4Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total YY1 protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu186 of human YY1 protein. Background YY1 (Yin Yang1) is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. YY1 contains four zinc finger motifs of the Cys-Cys-His-His type and can activate different eukaryotic genes (such as CREB, c-myc, Histone H4, p53 and PARP-1) or repress different eukaryotic genes (such as α-actin, IFN-β and IFN-γ) as well as regulate some viral promoters (1). YY1 deficient embryos die approximately at the time of implantation, suggesting that YY1 has an essential role in embryonic development (2). YY1 is overexpressed in cancer cells such as prostate cancer and therefore may be considered a prognostic marker (1). Alternate Names DELTA; Delta transcription factor; GADEVS; INO80 complex subunit S; INO80S; NF-E1; Transcriptional repressor protein YY1; TYY1; UCRBP; Yin and yang 1; Yin and Yang 1 protein; yin yang 1; YIN-YANG-1; YY-1; YY1; YY1 transcription factor Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 65 Source/Isotype: Rabbit IgG