CST, 63246S, CD58 (F7R6O) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD58. Validated for WB,IP,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, CD58 (F7R6O) Rabbit Monoclonal Antibody (CST #63246) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400 Flow Cytometry (Live): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD58 (F7R6O) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD58 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD58 protein. Background CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a ubiquitously expressed glycoprotein anchored to the plasma membrane through a transmembrane domain or a GPI linkage (1). Research studies have demonstrated that CD58 plays a critical role in immune synapse formation by functioning as a co-stimulatory adhesion molecule upon engagement of the CD2 antigen on the surface of T cells and NK cells. As such, the CD58-CD2 axis facilitates the execution of immune effector cell functions, including activation, proliferation, and cytotoxicity (2-4). In the context of cancer, recent studies have pointed to the CD58-CD2 axis as a stimulatory immune checkpoint, and lesions to this axis compromise anti-tumor immune responses (5,6). Alternate Names Ag3; CD58; CD58 antigen, (lymphocyte function-associated antigen 3); CD58 molecule; LFA-3; LFA3; Lymphocyte function-associated antigen 3; Surface glycoprotein LFA-3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 55-70 Source/Isotype: Rabbit IgG