CST, 63269T, VIP (D8J1V) Rabbit Monoclonal Antibody (IF Formulated)

Monoclonal Antibody for studying VIP. Validated for Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, VIP (D8J1V) Rabbit Monoclonal Antibody (IF Formulated) (CST #63269) is ready to ship. Product Usage Information Immunofluorescence (Frozen): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Frozen) Specificity / Sensitivity VIP (D8J1V) Rabbit Monoclonal Antibody (IF Formulated) recognizes endogenous levels of total VIP protein. This antibody is specifically formulated for the detection of total VIP protein in fixed-frozen tissue sections using standard indirect fluorescent detection. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn133 of human VIP protein. Background Vasoactive intestinal polypeptide (VIP) is a neuropeptide synthesized as a precursor that is processed to an active mature peptide of 28 residues (1). VIP is produced by neurons, endocrine, and immune cells and is expressed in many tissues, in agreement with its various biological functions (2). VIP acts through activation of two receptors belonging to the G protein-coupled receptor family, VPAC1 and VPAC2 (2) and elicits several effects such as vasodilation, regulation of smooth muscle cell contractility, and blood flow in the gastrointestinal track (3,4). In addition, VIP is involved in the regulation of T cell differentiation (6), and in immunosuppression (7,8). Alternate Names Intestinal peptide PHM-27; Intestinal peptide PHV-42; MGC13587; Peptide histidine methioninamide 27; Peptide histidine valine 42; PHM27; prepro-VIP; Vasoactive intestinal peptide; Vasoactive intestinal polypeptide; VIP; VIP peptides Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG