CST, 6327S, SignalSilence® IkappaB alpha siRNA I

siRNA for studying IkB-a in the research area. Product Usage Information CST recommends transfection with 100 nM SignalSilence ® IkappaB alpha siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well. Storage SignalSilence ® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC. Background The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8). Alternate Names IκB-α; IκBα Specification REACTIVITY: H