CST, 63290S, p47phox (F3Y6C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying p47phox. Validated for WB,WB,IHC,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, p47phox (F3Y6C) Rabbit Monoclonal Antibody (CST #63290) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 IHC Leica Bond: 1:2000 - 1:8000 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Immunofluorescence (Immunocytochemistry): 1:200 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 31169 . Protocol Available protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity p47phox (F3Y6C) Rabbit Monoclonal Antibody recognizes endogenous levels of total p47phox protein. This antibody is not approved for immunofluorescence on frozen mouse tissue due to low sensitivity. This antibody detects a 30 kDa band of unknown origin in some samples. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala227 of human p47phox protein. Background The phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The NADPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformational change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558 (1). Defects in p47phox, often resulting from recombination between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease (2-4). Elevated oxidative stress due to increased myocardial NADPH oxidase activity may be a contributing factor in heart failure (5,6). Alternate Names 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; CGD1; FLJ79451; NADPH oxidase organizer 2; NCF-1; NCF-47K; NCF1; NCF1A; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; NOXO2; p47-phox; p47phox; SH3 and PX domain-containing protein 1A; SH3PXD1A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG