CST, 63388SF, Huntingtin (F8E9N) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Huntingtin. Validated for WB,IHC,IF,IF. Highly specific and rigorously validated in-house, Huntingtin (F8E9N) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #63388) is ready to ship. Product Usage Information This product is the carrier free version of product #73232. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 73232 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Huntingtin (F8E9N) Rabbit Monoclonal Antibody recognizes endogenous levels of total huntingtin protein. This antibody is not recommended for immunohistochemical analysis of human tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human huntingtin protein. Background Huntington's Disease (HD) is a fatal neurodegenerative disorder characterized by psychiatric, cognitive, and motor dysfunction. HD neuropathology involves the selective degeneration of specific neuronal subpopulations, including GABA-ergic neurons of the striatum and those within the cerebral cortex (1,2). The genetic analysis of HD has been the flagship study of inherited neurological diseases, from initial chromosomal localization to identification of the gene. Huntingtin is a large (340-350 kDa) cytosolic protein that may be involved in a number of cellular functions such as transcription, gastrulation, neurogenesis, neurotransmission, axonal transport, neural positioning, and apoptosis (2,3). The gene from unaffected individuals contains between 6 and 34 CAG trinucleotide repeats, with expansion beyond this range causing the onset of disease symptoms. A strong inverse correlation exists between the age of onset in patients and the number of huntingtin gene CAG repeats encoding a stretch of polyglutamine peptides (1,2). The huntingtin protein undergoes numerous post-translational modifications, including phosphorylation, ubiquitination, sumoylation, palmitoylation, and cleavage (2). Phosphorylation of Ser421 by Akt can partially counteract the toxicity that results from the expanded polyglutamine tract. Varying Akt expression in the brain correlates with regional differences in huntingtin protein phosphorylation; this pattern inversely correlates with the regions that are most affected by degeneration in diseased brain (2). A key step in the disease is the proteolytic cleavage of huntingtin protein into amino-terminal fragments that contain expanded glutamine repeats and translocate into the nucleus. Caspase-mediated cleavage of huntingtin at Asp513 is associated with increased polyglutamine aggregate formation and toxicity. Phosphorylation of Ser434 by CDK5 protects against cleavage (2,3). Alternate Names HD; HD protein; HTT; Huntingtin; Huntingtin, myristoylated N-terminal fragment; Huntington disease protein; IT15; LOMARS Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 350 Source/Isotype: Rabbit IgG