CST, 63435SF, ATF-4 (E4Q4E) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ATF-4. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, ATF-4 (E4Q4E) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #63435) is ready to ship. Product Usage Information This product is the carrier free version of product #97038. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 97038 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ATF-4 (E4Q4E) Mouse Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ATF-4 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro134 of human ATF-4 protein. Background ATF-4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway (1-3). ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4 (2). Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress (4). Alternate Names Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); ATF-4; ATF4; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2; CREB2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67; TXREB Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Mouse IgG1 kappa