CST, 63445T, UFM1 (F9J1A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying UFM1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UFM1 (F9J1A) Rabbit Monoclonal Antibody (CST #63445) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UFM1 (F9J1A) Rabbit Monoclonal Antibody recognizes endogenous levels of total UFM1 protein. Higher molecular weight bands are expected as a result of UFM1 conjugation. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp53 of human UFM1 protein. Background The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4). Alternate Names bA131P10.1; BM-002; C13orf20; HLD14; ubiquitin fold modifier 1; Ubiquitin-fold modifier 1; UFM1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 10 Source/Isotype: Rabbit IgG