CST, 63523S, Phospho-A20/TNFAIP3 (Ser381) Antibody

Polyclonal Antibody for studying TNFAIP3 (Ser381) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-A20/TNFAIP3 (Ser381) Antibody (CST #63523) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-A20/TNFAIP3 (Ser381) Antibody recognizes endogenous levels of A20/TNFAIP3 protein only when phosphorylated at Ser381. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser381 of human A20/TNFAIP3 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background A20, also referred to as TNF-α-induced protein 3 (TNFAIP3), is cytokine-inducible protein that functions to inhibit apoptosis and activate NF-κB (1,2). It was first identified as a TNF-α inducible primary response gene in human umbilical vein endothelial cells, and encodes a 790-amino acid protein containing seven Cys2/Cys2-zinc finger motifs (3). Constitutive expression of A20 is observed in lymphoid tissues (4), but it is transiently expressed in a variety of cell types in response to inflammatory signals such as TNF-α (3,5), IL-1 (3,5), phorbol esters (6), and LPS (7). Expression of A20 can confer resistance to apoptosis and NF-κB activation triggered by these signals, probably through interference with TRAF (TNF receptor associated factor) family members (8,9), and interaction with the NF-κB inhibiting protein ABIN (10). Studies also show that A20 contains site-specific ubiquitin modifying activity that can contribute to its biological functions (11,12). The amino-terminus of A20 contains de-ubiquitinating (DUB) activity for Lys63 branches, such as those found in TRAF6 and RIP, while the carboxyl-terminus contains ubiquitin ligase (E3) activity for Lys48 branches of the same substrates and leads to their degradation (12). A20 gets phosphorylated at Ser381 by IKKβ downstream of various innate immune activators, which enhances the deubiquitnase activiaty of A20 leading to disassembly of inflammatory signaling complexes (13). Therefore, A20 works as part of a negative feedback loop to limit NF-κB activation and inflammatory signaling (13). Phosphorylation at Ser381 could be used as a marker of active A20. Alternate Names A20; A20p37; A20p50; AISBL; MGC104522; MGC138687; MGC138688; OTU domain-containing protein 7C; OTUD7C; Putative DNA-binding protein A20; TNAP3; TNF alpha induced protein 3; TNF alpha-induced protein 3; TNFA1P2; TNFAIP3; Tumor necrosis factor alpha-induced protein 3; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha induced protein 3; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 92 SOURCE: Rabbit