CST, 63528SF, gamma-Catenin (D9M1Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Catenin-gamma. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, gamma-Catenin (D9M1Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #63528) is ready to ship. Product Usage Information This product is the carrier free version of product #75550. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 75550 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity gamma-Catenin (D9M1Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total γ-Catenin protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro725 of human γ-Catenin protein. Background Also known as plakoglobin, γ-catenin is a member of the Armadillo family of signaling molecules, which includes β-catenin and the protein armadillo (1). This family of proteins is involved in Wnt signaling, which is important in embryonic development and in tumorigenesis (2-3). Although the two vertebrate proteins β- and γ-catenin display sequence homology, γ-catenin likely plays a role distinct from that of β-catenin (1, 4-6). γ-catenin localizes to desmosomes and adherens junctions, both sites of intercellular adhesion, and interacts with the cytoplasmic domains of classical and desmosomal cadherins. Interaction of γ- or β-catenin with α-catenin, desmoplakin and other junction proteins provides a link between intercellular junctions and the actin and intermediate filament cytoskeleton. Maintenance and/or modification of this link is vital for control of cell adhesion and migration (1). γ-catenin is modified by phosphorylation, affecting both adhesion and β-catenin dependent transcription (7), and by and O-glycosylation, affecting adhesion (8). Recent evidence suggests that γ-catenin regulates desmosomal adhesion in response to growth factor stimulation (9). Alternate Names ARVD12; catenin (cadherin-associated protein), gamma (80kD); catenin (cadherin-associated protein), gamma 80kDa; Catenin gamma; CTNNG; Desmoplakin III; Desmoplakin-3; DP3; DPIII; gamma-catenin; Junction plakoglobin; JUP; PDGB; PKGB; PLAK Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 83 Source/Isotype: Rabbit IgG