CST, 63633S, BCAP (E1U9X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BCAP. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BCAP (E1U9X) Rabbit Monoclonal Antibody (CST #63633) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BCAP (E1U9X) Rabbit Monoclonal Antibody recognizes endogenous levels of total BCAP protein. This antibody reacts with BCAP-L and BCAP-S protein isoforms. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human BCAP protein. Background B cell adaptor for PI3K (BCAP) is a multidomain-containing signaling adaptor that is expressed by myeloid cells, B cells, and NK cells (1,2). Two major BCAP protein isoforms that differ significantly in size, BCAP-L and BCAP-S, have been shown to be expressed by macrophages, B cells, and NK cells (1,2). Although it remains unclear if there is a differential function for these isoforms, both BCAP-L and BCAP-S undergo robust tyrosine phosphorylation by multiple tyrosine kinases in response to B cell receptor and Toll-like receptor engagement, which promotes association of BCAP with multiple SH2- and SH3-domain-containing proteins (1,3,4). In the context of B cells, research studies have demonstrated that phosphorylation of the YXXM motifs within BCAP are critical for the recruitment and activation of PI3K to drive B cell proliferation (4,5). Macrophages, however, utilize the BCAP/PI3K signaling module to restrain proinflammatory functions in response to infection (3,6). Alternate Names 1810044J04Rik; B cell adaptor protein; B-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1; BCAP; Phosphoinositide 3-kinase adapter protein 1; phosphoinositide-3-kinase adaptor protein 1; PIK3AP1; RP11-34E5.3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 105, 98, 85, 78 Source/Isotype: Rabbit IgG