CST, 63647S, TIAM1 Antibody

Polyclonal Antibody for studying TIAM1. Validated for Western Blotting. Highly specific and rigorously validated in-house, TIAM1 Antibody (CST #63647) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TIAM1 Antibody recognizes endogenous levels of total TIAM1 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TIAM1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background TIAM1 (T-lymphoma invasion and metastasis-inducing protein 1) is a multidomain guanine nucleotide exchange factor (GEF) protein that activates Rac1, a GTPase involved in cytoskeletal dynamics that regulate cell migration, growth, and survival. TIAM1 also has been identified as an inhibitor of the YAP/TAZ signaling pathway, with two distinct subcellular mechanisms of action: (1) promoting cytoplasmic (proteosomal) degradation of YAP and TAZ; and (2) blocking the transcriptional co-activator functions of YAP and TAZ in the nucleus (3,4). The effects of TIAM1 on tumor development are also complex and context-dependent. For example, it has been reported that TIAM1 can promote tumor growth and progression in some contexts, while antagonizing tumor metastasis and invasion in other contexts (5,6). Alternate Names FLJ36302; human T-lymphoma invasion and metastasis inducing TIAM1 protein; Rho guanine nucleotide exchange factor TIAM1; T cell lymphoma invasion and metastasis 1; T-cell lymphoma invasion and metastasis 1; T-lymphoma invasion and metastasis-inducing protein 1; TIAM Rac1 associated GEF 1; TIAM-1; TIAM1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 220 SOURCE: Rabbit