CST, 6364S, COUP-TFI (D4H2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying COUP-TF1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, COUP-TFI (D4H2) Rabbit Monoclonal Antibody (CST #6364) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity COUP-TFI (D4H2) Rabbit Monoclonal Antibody recognizes endogenous levels of total COUP-TFI protein. This antibody does not cross-react with COUP-TFII protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln165 of human COUP-TFI protein. Background Chicken ovalbumin upstream promoter transcription factor (COUP-TF) belongs to the NR2 subfamily of the nuclear hormone receptor family (1). COUP-TFI and COUP-TFII are two of the well-characterized members in the NR2 subfamily. These two members are highly conserved in their two zinc-finger DNA binding domains (DBD) and the ligand binding domain (LBD), and function as repressors or activators of downstream target genes to regulate different biological processes (1-3). COUP-TFI and II bind to 5'-AGGTCA-3' motif palindromes, either directly or indirectly, through heterodimer formation with other proteins (e.g. RXRs) to regulate downstream target gene expression (4,5). COUP-TFI is involved in neuronal development, tissue patterning, and differentiation (6-8). COUP-TFII has been shown to be involved in angiogenesis, glucose homeostasis, and mesenchymal cell commitment (9-12). Alternate Names BBOAS; BBSOAS; chicken ovalbumin upstream promoter-transcription factor I; COT1; COUP transcription factor 1; COUP transcription factor I; COUP-TF I; COUP-TF1; COUP-TFI; COUPTF1; EAR-3; EAR3; ERBAL3; NR2F1; NR2F2; Nuclear receptor subfamily 2 group F member 1; nuclear receptor subfamily 2, group F, member 1; SVP44; TCFCOUP1; TFCOUP1; transcription factor COUP 1 (chicken ovalbumin upstream promoter 1, v-erb-a homolog-like 3); V-erbA-related protein 3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 47 Source/Isotype: Rabbit IgG