CST, 63660S, AIM2 Antibody

Polyclonal Antibody for studying AIM2 mouse. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, AIM2 Antibody (CST #63660) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity AIM2 Antibody recognizes endogenous levels of total AIM2 protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val104 of mouse AIM2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Absent in melanoma 2 (AIM2) is an interferon-inducible protein containing an amino-terminal pyrin domain and carboxy-terminal HIN-200 domain that functions in innate immunity and tumor progression (1). Expression of AIM2 can inhibit cell growth and tumor formation (2,3). Furthermore, the gene has a high frequency of mutations associated with microsatellite-unstable colorectal cancers (4). AIM2 has a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. Caspase-1 activation is regulated by multi-protein complexes referred to as "inflammasomes" (5,6). Distinct inflammasome complexes have been described containing NLRP1/NALP1, NLRP3/NALP3, IPAF, and AIM2. The HIN-200 domain of AIM2 is responsible for binding to cytoplasmic double-stranded DNA, resulting in caspase-1 activation. (7-9). This inflammasome complex also involves binding of the pyrin domain of AIM2 to the CARD-domain protein ASC/TMS1, which then interacts directly with caspase-1. As a result, AIM2 has been demonstrated to be an important sensor for a number of different pathogens (10-12). Alternate Names absent in melanoma 2; Aim2; Gm1313; Ifi-210; Ifi2; Ifi210; Interferon-inducible protein 210; Interferon-inducible protein AIM2; Interferon-inducible protein p210 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 43 SOURCE: Rabbit