CST, 63684SF, XCR1 (D2F8T) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying XCR1. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, XCR1 (D2F8T) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #63684) is ready to ship. Product Usage Information This product is the carrier free version of product #44665. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 44665 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity XCR1 (D2F8T) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total XCR1 protein by IHC-P and transfected levels of XCR1 protein by Western. Staining of unknown specificity in peripheral nerves has been observed. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human XCR1 protein. Background X-C motif chemokine receptor 1 (XCR1, GPR5, CCXCR1), part of the G protein-coupled superfamily, is expressed by a subset of dendritic cells and acts as a receptor for chemokines XCL1 and XCL2 (1,2,4). XCR1-positive dendritic cells cross-present antigens to naïve CD8 T cells, priming them to become activated cytotoxic CD8 T cells (3-5). In mouse models, the XCL1-XCR1 signaling axis was shown to be involved in the formation of self-tolerance through the development of T cells within the thymus (6). Alternate Names CCXCR1; chemokine (C motif) receptor 1; chemokine (C motif) XC receptor 1; Chemokine XC receptor 1; G protein-coupled receptor 5; G-protein coupled receptor 5; GPR5; Lymphotactin receptor; X-C motif chemokine receptor 1; XC chemokine receptor 1; XCR1 Specification REACTIVITY: H SENSITIVITY: Endogenous (IHC-P), Transfected (W) MW (kDa): 35 Source/Isotype: Rabbit IgG