CST, 63685SF, Phospho-MARCKS (Ser167/170) (D13E4) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying MARCKS (Ser167/Ser170) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-MARCKS (Ser167/170) (D13E4) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #63685) is ready to ship. Product Usage Information This product is the carrier free version of product #8722. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8722 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Phospho-MARCKS (Ser167/170) (D13E4) Rabbit Monoclonal Antibody recognizes endogenous levels of MARCKS protein only when phosphorylated at Ser167 and Ser170. This antibody may also detect MARCKS mono-phosphorylated at Ser167. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser167 and Ser170 of human MARCKS protein. Background yristoylated lanine- ich - inase ubstrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5). Alternate Names 80K-L; 80K-L protein; FLJ14368; FLJ90045; MACS; MARCKS; MARCS; myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; myristoylated alanine-rich protein kinase C substrate; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); phosphomyristin; PKCSL; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 rodent, 80 human Source/Isotype: Rabbit IgG